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Samples were obtained using canine EDTA blood surplus after clinical testing. A single drop was placed on filter paper, using a standard plastic pipette, and left for 30 seconds. The paper was then placed in a box with a standardized light source, and photographed with an I Phone 6S. Four images were taken. Each sample also had concurrent manual PCV measurement performed according to WHO guidelines. Colour intensity was measured using Image J in the largest homogenous area of the drop, the mean value from the four images was recorded. A scatter plot was generated using the obtained data, plotting the actual PCV against the average colour intensity. An additional ten samples were then evaluated with their colour intensity value inserted into the equation of the line generated, using the line of best fit.

Does the colour intensity of a blood spot image obtained by smartphone accurately correlate with canine packed cell volume (PCV)