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The undesirable traits generally referred as "Off-type", in F1 generations is unacceptable beyond certain percentage for commercially cultivated crops. The traditional method of Grow-Out-Test is time consuming and laborious. Identification of the off-types with a Simple and powerful molecular marker tool before marketing is the need of the hour. In this present study the off-types are identified using SSR markers for Chilli, Tomato, Okra, cucumber and Bitter gourd. Collectively 96 crop specific SSR markers were screened for one variety from each crop and one polymorphic primer was identified for each parental line of all the crops. These polymorphic primers were validated on hybrids and the off-types were identified based on the banding pattern in comparison with the polymorphic allelic band of parents. These identified off-types were further allowed to grow completely till fruiting and the variation in fruit and leaves characteristic proved it be an off-types when compared with actual hybrid. So, SSR markers can be used as a quick and accurate means as a molecular marker tool for validating the commercially cultivated crops, which saves time and labor for commercial seed companies.

Identification of off-type in F1 Hybrids of Commercially Cultivated Vegetable Crops using SSR Markers as a Molecular Tool