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Ricinus communis stem is one of the potential substrate for bioethanol production; however outer lignin sheath is a major bottleneck for saccharification and so to the fermentation. The conventional acid based pretreatment has major drawback as it produced inhibitors like 5-hydroxy methyl furfural (HMF) and furfural. Present investigation is attempted to evaluate delignification by treatment of alkaline peroxide and laccase. Delignification and saccharification are studied and compared on the basis of weight loss of biomass during the process, lignin loss, and reducing sugar yield after cellulase treatment. Alkaline peroxide treatment showed around 34% weigh loss of biomass and 8.26% of reduced lignin in a process, as compared to native biomass (20.60%). Saccharification yield was found as reducing sugar 774.48 mg/g dry weight pretreated biomass. In case of laccase, results were recorded as 23% weight loss, 11.50% lignin remains after treatment and 658.67 mg/g reducing sugar as saccharification yield.

Alkaline Peroxide and Laccase Treatment for the Delignification of Ricinus Communis Biomass to Enhance Saccharification