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Soil samples from different locations were collected and the amidase producing organisms were isolated by enrichment culture method in soil extract medium using acetamide as sole source of C and N and incubating at 37°C. A total of 29 bacterial isolates were obtained. Eight strains showed amidase production. The highest amidase producing strain (HSS22) was revealed as Paenibacillus polymexa. Acetamide containing mineral salt media when supplemented with Sodium citrate for P. polymexa resulted in higher growth of bacteria but addition of NaCl did not show remarkable effect on bacterial growth, this indicated that amides could be efficiently utilized as N source. Isolate P. polymexa was found to show highest amidase activity (19.34 U/ml) among the nine amidase producing isolates. The pH tolerance, temperature and amidase production capabilities of P. Polymexa were compared. It was found that P. polymexawas able to grow at temperature of 37°C, the pH 8.0, whereas another six isolates showed amidase activity in the pH range of (6.0-9.0) and temperature (30°C) thus P. polymexawas selected for subsequent studies. The results for enzyme activity under the performance of different physiochemical conditions and purification as well as molecular weights are recorded and discussed.

Isolation, Characterization and Activity of Amidase Producing Paenibacillus Polymexa from Semi Arid Soils