English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Isolated adult rat hepatocytes attached to flasks in the presence of frbronectin, spread, formed monolayer, and subsequently proliferated in Koga’s medium L under serum-free conditions. When a plasmin inhibitor, aprotinin (Trasylol) was omitted from the medium, hepatocytes tended to shrink after initial spreading, were detached from the substrata and died within 2 or 3 days. Examination of the hepatocyte-conditioned medium revealed the presence of plasmin-like protease activity. Since other plasmin inhibitors also prevented hepatocytes from shrinking and detachment, it is suggested that plasmin-like protease(s) is produced by hepatocytes and this causes detachment of hepatocytes. Thus, it is necessary to supplement a plasmin inhibitor such as aprotinin for successful cultivation of hepatocytes under serum-free conditions. We have previously developed Koga’s medium L (7, ll, 12) for cultivation ofadult rat hepatocytes under serum-free conditions and succeeded in inducing both DNA synthesis and mitosis. Characteristic features of this medium are the presence of a high concentration of Na-pyruvate (5), a low concentration of Ca”, proline (7) and aprotinin (bovine pancreatic trypsin inhibitor, Trasylol) (6). In our initial study, we used plasmin digest of commercial fibrinogen, in which aprotinin was added to inhibit plasmin activity, to promote cell attachment and spreading (l2). Later, we found that the presence of aprotinin was essential for successful cultivation of hepatocytes under serum-free conditions (6). The importance of aprotinin was recently supported by other investigators (l). In this study, we examined the role of aprotinin and other plasmin inhibitors on growth of hepatocytes in serum-free culture. MATERIALS AND METHODS

<b>ROLE OF PLASMIN INHIBITORS IN GROWTH OF ADULT RAT HEPATOCYTES IN SERUM-FREE PRIMARY </b><b>CULTURE </b>