English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

A model of dermis was prepared by embedding rat dermal fibroblasts into collagen lattices (27). A skin model was reconstituted by overlaying the dermal model with rat epidermal cells and was maintained in culture. Both fibroblasts and epidermal cells showed intense collagenolytic activity. This collagenolysis was completely inhibited by a specific inhibitor of animal collagenase. Cortisol effectively inhibited the lytic activity of these cells. Collagenolysis in the skin model was expressed earlier than that of dermal model or that of epidermal cells alone and was more intense than the sum of collagenolysis of the dermal model and the epidermal cells. Furthermore, epidermal cells spread much better on the dermal model than on the fibroblasts-free collagen gel. These facts indicate the presence of interactions between fibroblasts and epidermal cells. Desmosomes were recognized electron microscopically around the epidermal cells in the model. Hemidesmosomes, however, were not formed. Tonofilaments were scattered in the cytoplasm and did not show an organized orientation. Presence of laminin and fibronectin was confirmed immunohistochemically at the boundary of epidermal cells and dermis within a week of culture of the skin model. However, the skin model failed to form the structure of a basal lamina. Owing to current development in methodconnective tissue cells and extracellular maology of isolation and cultivation of various types of cells from higher animals, it is now possible to reconstitute models of organs or tissues which mimic the structures and functions of the in vivo counterparts. Such a model can be constructed by mixing epithelial cells, 3Present address: The Japan Biomedical Material Research Center, Meguro, Tokyo 152, Japan 5Present address: Department of Ultrastructural Research, Kitasato University School of Nursing, Sagamihara, Kanagawa 228, Japan trices in an organized fashion and will be useful for obtaining information on interactions of both cell to cell and cell to matrix components. These interactions might contribute to the development and stability of organs and tissues in the body. Bell er al. published a pioneering work on reconstitution of a skin model and succeeded in grafting it as a skin-equivalent tissue of full thickness (3). We thought that this type of a reconstituted in virro model of the skin might be useful to investigate cellular regulation of structural organization of the tissue and

<b>A RECONSTITUTED SKIN: DERMAL-EPIDERMAL INTERACTIONS IN COLLAGENOLYSIS AND CELL </b><b>MORPHOLOGY </b>