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Glutathione S-transferases (GSTs) were purified from rat brain cytosol by a procedure including Sephadex G-75, glutathione-linked Sepharose 6B and CM-cellulose column chromatography. Three enzyme fractions were obtained by column chromatography on CM-cellulose; the fractions were apparently homogeneous as judged by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Its native form was a dimeric protein with a molecular weight of 45,000, and chromatofocusing of the enzymes gave four peaks at pl 6.7, 7.6, 8.9 and 10.1. Antibodies raised against GSTs ofrat liver cytosol were used to identify the purified enzymes. It has been demonstrated that rat brain cytosol has no ligandin (GST-L2=YaYa_), which is the major component of liver GST. Glutathione S-transferases (GSTs; EC 2.5.1.18) are important enzymes for detoxication because of their ability to conjugate a variety of electrophilic compounds with glutathione (9). The highest GST activity is present in the liver and detailed studies have been performed (9). Recent studies have revealed the pesence of the enzyme in mammalian and avian brain (5, 17). Although it has been reported that brain GST binds with acrylamide, a neurotoxic agent (7), and the enzyme can be significantly induced by a prolonged treatment with phenobarbital or 3methylcholantrene (4), the physiological role of the enzyme has not been fully elucidated (7). Our recent immunohistochemical study has shown that GSTs are exclusively localized in astrocytes and ependymal cells, but not in oligodendrocytes and neurons, of adult rat brain and they are especially abundant in the nucleus (14). The present study was undertaken to evaluate ‘To whom all correspondence should be addressed the structural properties ofbrain GST and it was found that rat brain cytosol contained no ligandin (GST-L2=YaYa), which is known to be the major component of GST in liver (8). MATERIALS AND METHODS

biomedical research

&lt;b&gt;PURIFICATION AND CHARACTERIZATION OF &lt;/b&gt;&lt;b&gt;GLUTATHIONE &lt;i&gt;S&lt;/i&gt;-TRANSFERASE FROM RAT BRAIN &lt;/b&gt;&lt;b&gt;CYTOSOL: IDENTIFICATION OF FOUR ISOZYMES AND EVIDENCE FOR ABSENCE OF THE Ya SUBUNIT &lt;/b&gt;