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Cell attachment to chemically defined substrata was studied with mouse 3T6 cells which were seeded on glass dishes coated with syndiotactic poly(2-hydroxyethyl methacrylate), isotactic poly(ethyl methacrylate), or syndiotactic poly(ethyl methacrylate). Cells suspended in serum-free medium attached to both the isotactic and syndiotactic poly(ethyl methacrylate)-substrata but not to the syndiotactic poly(2-hydroxyethyl methacrylate)substratum. On the other hand, cells suspended in serum-containing medium did not attach to any of the three substrata, and the inhibition of cell attachment was correlated with the dose of polymers added to the glass dishes. The dose-response experiment showed that there was a little, but significant, difference between isotactic and syndiotactic poly(ethyl methacrylate)s. The results indicate that the substitution of hydrogen for the hydroxyl group in poly(2-hydroxyethyl methacrylate) results in notable modifications of substratum adhesivity; the inhibition of cell attachment to poly(2-hydroxyethyl methacrylate) is independent of serum components whereas the inhibition of cell attachment to poly(ethyl methacrylate) is dependent on serum components, and syndiotactic poly(ethyl methacrylate) more sharply disturbs cell attachment than does the isotactic polymer. Cell attachment to appropriate substrata is profoundly associated with the physiological conditions of the cells such as growth (25) and viability (21). Substrata modified with poly(2-hydroxyethyl methacrylate) (PHEMA) and poly(methyl methacrylate) (PMMA) have been widely utilized in various experiments to obtain desired adhesivity for cell attachment. These studies included investigations on cell growth (l, 4, 7, 25), macromolecular metabolism (2), and expression ofa differentiated property (3, 18). Their aim was to find the desired conditions for substratum (anchorage) efficiency which is controlled by the dose of PHEMA. In this study, three species of po1ymethacrylate, i.e. syndiotactic poly(2-hydroxyl methacrylate) (syn-PHEMA), syndiotactic poly(ethyl methacrylate) (syn-PEMA) and isotactic poly(ethyl methacrylate) (iso-PEMA) were used. The principal aim of this study was to determine the availability of the three polymers and to investigate the different responses of cultured cells, mouse 3T6 cells, which are well characterized. To the best of our knowledge, this type of approach has never been tried. It was found that the substitution of hydrogen for the hydroxyl group and the changes in tacticity in polymethacrylate results in notable modifications of substratum adhesivity. MATERIALS AND METHODS Cell Line and Culture Conditions The 3T6 mouse fibroblast cell line (28) was used in all the experiments. The cells were subcultured in Dulbecco’s modified Eagle’s medium

<b>POLYMERS OF METHACRYLATE AVAILABLE FOR OBTAINING VARIETIES OF CELL-SUBSTRATUM ADHESIVITY</b>