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Localization of the gastrin-releasing peptide (GRP)-like immunoreactivity in the brain ofthe Japanese quail pretreated with colchicine was investigated using anti-porcine GRP serum and anti-bombesin serum. In the hypothalamus, numerous neurons exhibiting GRP-immunoreactivity were detected in the suprachiasmatic nucleus. In the extrahypothalamus, GRP neurons were widely distributed in the telencephalon (hyperstriatum accessorium, hyperstriatum ventrale, neostriatum dorsolaterale, neostriatum caudale, archistriatum and lobus parolfactorius), and also in the mesencephalon (caudal part of the stratum cellulare internum, ectomamillary nucleus, formatio reticularis medialis mesencephali and substantia grisea centralis). The GRP-immunoreactive fibers were seen in the nucleus ofstria terminalis oftelencephalon and in the nucleus infundibularis of the hypothalamus, where they were in close contact with the unlabeled perikarya. With the anti-bombesin serum, neurons and fibers were not immunostained through-

biomedical research

&lt;b&gt;LOCALIZATION OF GASTRIN-RELEASING PEPTIDE (GRP)LIKE IMMUNOREACTIVITY IN THE BRAIN OF THE JAPANESE QUAIL, &lt;i&gt;COTURNIX &lt;/i&gt;&lt;/b&gt;&lt;b&gt;&lt;i&gt;COTURNIX &lt;/i&gt;&lt;/b&gt;