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Telomerase activity plays an important role in the regulation of cell senescence by limiting the number of divisions. Significant levels of telomerase activity have been detected in 85% to 90% of human cancers, however none or negligible amounts of telomerase activity were observed in benign tumors and healthy tissue. This study investigated the levels of telomerase activity in three cancer cell lines and a non-tumorigenic cell line after exposure to two endogenous metabolites respectively, namely 2-methoxyestradiol (2ME) and prostaglandin A 2 (PGA 2 ). Cells were exposed to either 1 μM 2ME or 20 μg/ml PGA 2 and their relative telomerase activity (RTA) was measured by means of a combined method including the polymerase chain reaction and an enzyme-linked immunosorbent assay. PGA 2 exposure caused a statistically significant decrease in the RTA of the three cancer cell lines (HeLa, WHCO3, MCF-7) (P < 0.05). In contrast, the RTA of the non-tumorigenic MCF-12A cell line was statistically significantly increased after exposure to PGA2 (P < 0.00005). The RTA of HeLa, WHCO3 and MCF-12A cells were significantly decreased after 2ME exposure (P < 0.05). These results confirm that PGA 2 and 2ME could be of potential value as antitumor agents, since both exert an inhibitory activity on the tumour-associated function of the telomerase enzyme involved in cancer cell immortality.

Influence of Prostaglandin A2 and 2-Methoxyestradiel on Telemerase Activity in Cancer Cell Lines and a Non-tumorigenic Epithelial Breast Cell Line