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We have isolated the human angiotensin II (AII) type I receptor (AT 1 ) gene,whose coding region was contained within a single exon and have established Chinese hamster ovary (CHO) cell lines stably expressing this recombinant receptor. The expressed receptor revealed the typical binding characteristics of the AT 1 receptor to several AII receptor agonists and antagonists. Treatment of the intact CHO cells with the sulfhydryl reducing reagent dithiothreitol predominantly decreased the radioligand binding, indicating that a disulfhydryl bond(s) necessary for AII binding is located on extracellular domains of the receptor molecule

biomedical research

&lt;b&gt;ESTABLISHMENT OF CHINESE HAMSTER OVARY CELL LINES STABLY EXPRESSING THE CLONED HUMAN TYPE 1 ANGIOTENSIN II RECEPTOR AND CHARACTERIZATION OF THE EXPRESSED &lt;/b&gt;&lt;b&gt;RECEPTOR &lt;/b&gt;