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Aim  We sought to characterize the contribution of the  O -OTase, PglL, to virulence in two  Burkholderia  spp. by comparing isogenic mutants in  Burkholderia pseudomallei  with the related species,  Burkholderia thailandensis .    Materials & methods  We utilized an array of  in vitro  assays in addition to  Galleria mellonella  and murine  in vivo  models to assess virulence of the mutant and wild-type strains in each  Burkholderia  species.    Results  We found that  pglL  contributes to biofilm and twitching motility in both species.  PglL  uniquely affected morphology; cell invasion; intracellular motility; plaque formation and intergenus competition in  B. pseudomallei . This mutant was attenuated in the murine model, and extended survival in a vaccine-challenge experiment.    Conclusion  Our data support a broad role for  pglL  in bacterial fitness and virulence, particularly in  B. pseudomallei .

Virulence of the emerging pathogen, Burkholderia pseudomallei, depends upon the O-linked oligosaccharyltransferase, PglL