Open AccessIntegrated Analysis of Transcriptome-Wide m 6 A Methylome of Osteosarcoma Stem Cells Enriched by Chemotherapy
Author(s) -
Yongjie Wang,
Lingling Zeng,
Chao Liang,
Rui Zan,
Weiping Ji,
Zhichang Zhang,
Yuxuan Wei,
Shikui Tu,
Yang Dong
Publication year - 2019
Publication title -
epigenomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.265
H-Index - 60
eISSN - 1750-192X
pISSN - 1750-1911
DOI - 10.2217/epi-2019-0262
Subject(s) - biology , transcriptome , osteosarcoma , western blot , stem cell , methylation , dna methylation , gene , cancer research , gene expression , microbiology and biotechnology , genetics
Aim: To analyze the m 6 A methylome of osteosarcoma stem cells (OSCs). Materials & methods: Chemoresistant OSCs were enriched by doxorubicin treatment. Expression of m 6 A-related enzymes was detected by quantitative real-time-PCR and western blot. MeRIP-seq and RNA-seq were performed to identify differences in m 6 A methylation and gene expression. Data analysis was conducted to explore the modified genes and their clinical significance. Results: Three m 6 A-related enzymes were altered in OSCs. Differentially methylated genes were enriched in some pathways regulating pluripotency of stem cells. The expression of several candidate genes were found consistent with that in GSE33458 dataset, and associated with poor prognosis in osteosarcoma patients. Conclusion: m 6 A may play a role in the emergence and maintaining of OSCs and affect the prognosis.
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