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Plants have mechanisms for accumulation, tolerance or alleviation of high levels of heavy metals in contaminated soil. Some contaminants can be absorbed by the plants and are then broken down by plant enzymes. The objective of this particular study was measure the potential activities of three enzymes (dehydrogenase, protease and phosphatase) in the alfalfa rhizospheres under the stress at different concentrations of cadmium salts, and inoculated different biofertilizers strains of S. meliloti and coinoculated with Trichoderma strains. As well as pH, NaCl, CaCO3 tolerance and antibiotic resistance were investigated. The results show that the growth rate of sinorhizobial strains decreased with increasing of NaCl and CaCO3 concentration. Sinorhizobial strains grew in environments of pH ranged between5.5-7.5. There was variable response to antibiotic of all sinorhizobial strains. As well it was clearly showed that Cdso4 reduced the activity of phosphatase. Trichoderma harzianum stimulate the enzyme activity more than Trichoderma viride. Keywords— Alfalfa, Heavy metals, pH condition, Rhizobioremediation.

Effects of Cd on Microbial Properties, Enzymatic Activities, Soil pH and Salinity in The Rhizosphere of Medicago sativa