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Rebaudioside A and stevioside are abundant steviol glycoside contained in Stevia rebaudiana leaves. These components are widely used as a natural sweetener. The objective of this study was to develop rapid determination method of stevioside, and rebaudioside A in S. rebaudiana leaves using near infrared trans-reflectance spectroscopy (NIRS) combined with multivariate analysis. The reference method used was HPLC. A prediction model was developed using partial least square (PLS) regression. Calibration parameters were calculated based on a calibration set of various stevioside, rebaudioside A from 23 samples. Performance of PLS model was assessed in term of optimum determination coefficient (R 2 ), and minimum root mean square error of cross-validation (RMSEV). Validation of PLS model was performed using cross-validation and leave one out calibration of PLS component. Rebaudioside A has well PLS model in wavenumber region of 4100–5100 cm -1 , and stevioside determination using difference wavenumber region of 4760-5016 cm -1 . PLS model for total (sum of stevioside and rebaudioside A content) was exploited in wavenumber region of 4568-4928 cm -1 . NIRS in combination with multivariate data analysis of PLSR can be applied as a rapid method for determining rebaudioside A and the total amount of steviol glycosides in S. rebaudiana leaves .

indonesian journal of chemistry

Determination of Stevioside and Rebaudioside A in &lt;i&gt;Stevia rebaudiana&lt;/i&gt; Bertoni Leaves Using near Infrared Spectroscopy and Multivariate Data Analysis