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A thermophilic aerobe microorganism designated NP4, was isolated from the heated sugar cane. It grew on dextran, and produced a thermoactive extracellular dextranase. Screening and isolation was done by assay of dextranase activity semi quantitatively on solid medium containing blue dextran . It provided several colonies with different morphology exhibited decolourized zones around, on culture plates containing blue dextran 2000 R . The screening resulted in isolation of one microbe which efficiently assimilate dextran as carbon source. Dextranase production from the choised strain in liquid medium was conducted at room temperature for 8 hours with shaking speed of 125 rpm. The dextranase enzyme showed optimum pH of 8 and optimum temperature of 60 o C. Keywords: thermophilic aerobe, sugar cane, dextranase activity .

A THERMOPHILIC MICROBE PRODUCING DEXTRANASE FROM HEATED SUGAR CANE