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Six fenugreek ( Trigonella foenum-graecum  L.) plant samples showing leaf curl disease symptoms were collected from Pantnagar, Uttarakhand state of India. The disease was successfully transmitted by whitefly ( Bemisia tabaci)  to healthy seedlings of fenugreek. PCR using begomovirus-specific primers amplified an expected 1.2-kb product of virus genome, and its determined sequence suggested that the six infected fenugreek plant samples were associated with previously described monopartite begomovirus, ageratum enation virus (AEV) (nucleotide identities is more 98% among themselves). Therefore, one sample (BG199) was selected for full-length genome (DNA-A) and subviral (beta satellite) DNA amplification using rolling circle amplification method. Sequence Demarcation Tool (SDT) analysis of the complete genome of the isolate BG199 and beta satellite showed highest nucleotide (nt) identity of 93.7-98.7% and 90.7-95.6% with AEV and ageratum yellow leaf curl beta satellite (AYLCB), respectively. Further analysis of the recombinant origin of the genome (DNA-A) and beta satellite showed a major part of their genome was likely to be originated from the recombination of previously reported begomoviruses and beta satellites infecting different crops resulting in the evolution of a new recombinant virus. This is the first report of AEV, and AYLCB beta satellite components associated with leaf curl disease of fenugreek from India.

Molecular characterization of ageratum enation virus and beta satellite associated with leaf curl disease of fenugreek in India