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Mycobacterium avium subspecies paratuberculosis (MAP) is the cau se of Johns disease also called paratuberculosis. This is economically one of the important infectious diseases in cattle and ruminant husbandry. This disease is manifested as granulomatosis entrocolitis, lymphadenitis and inflammation local lymphatic vessels. The typical sign of this disease is progressive loss of weight. Considering the importance of detection of this disease in this study, two methods, culture and PCR, were used for the identification of this microorganism .In this study 100 milk samples from apparently healthy cows and 100 milk samples respectively. The results of this study showed that culture and PCR analyses methods are important in the identification of the causes of this disease. Therefore, considering the frequency of the disease in the studied region, either of those methods can be used in the microorganism identification.

Detection of Mycobacterium avium subsp. paratuberculosis in Cow Milk Using Culture and PCR methods