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This paper presents an experimental study on the preparation of gram-negative bacteria microcapsules by spray drying. Effects of operating conditions on the bacteria survival rate were examined by single factor analysis. Through orthogonal experiment, appropriate conditions were determined as 160°C of the inlet air temperature, 12.5 ml/min of the feed flow rate, 1:9 of the ratio between gum arabic and maltodextrin, and 50 ml of fluid nutrient medium with the bacteria. The bacteria survival rate reaches 84.57% associated with above conditions. Examination of storage performance shows that after preserving in plastic film bag for 6 months under ambient temperature from 18°C to 30°C, quantity of the viable gram-negative bacteria was increased by 148%, which was significantly improved compared to those inoculated from laboratory.

Microencapsulation of Gram-Negative Bacteria by Spray Drying