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Objective - The present study was aimed to evaluate the antioxidant and free radical scavenging properties of Oxystelma esculentum (MEOE).Methods- The total antioxidant, reducing power, DPPH radical, superoxide anion radical, nitric oxide radical, hydroxyl radical, hydrogen peroxide scavenging and total phenol content in MEOE was carried out.Results - The total antioxidant activity of MEOE increases with increasing concentration. The reducing capability and free radical scavenging activity in DPPH also increased in a dose dependent manner. The MEOE was found to scavenge the superoxide generated by PMS/NADH/NBT system. Moreover, MEOE was found to inhibit the nitric oxide radical generated from sodium nitropruside. MEOE was also found to inhibit the hydroxyl radical generated by Fe3+/ascorbate/EDTA/H2O2 system. The MEOE scavenges the hydrogen peroxide in a dose dependent manner. The amount of total phenolic compounds was also determined.Conclusion - The results suggest that the methanol extract of Oxystelma esculentum proved its strong antioxidant activity and can be used as a natural antioxidant.

Evaluation of Antioxidant and Free Radical Scavenging Activities of Oxystelma esculentum in Various In Vitro Models