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Cell growth and recombinant phytase production under the control of the GAP promoter were studied in continuous cultivation experiments of Pichia pastoris.  Based on these studies, a simple kinetic model was established to predict biomass and enzyme production. Although parameters were mainly estimated using the results of continuous cultivation, the proposed model was able to successfully predict cell growth and phytase production in three different fed-batch cultivations of P. pastoris using limited glucose feeding.

Modeling of Phytase Production by Cultivation of Pichia pastoris Under the Control of the GAP Promoter