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Using 2D-PAGE and SDS-PAGE, we elucidated the structure of the organic matrix in chicken eggshell for the first time. The large variety of components contained implies that the eggshell formation may be controlled by several components. We believe that 2D-PAGE is a good tool for separating the heterogeneous organic matrix and can be used to analyze the organic matrix in different kinds of biominerals.

Two-dimensional gel electrophoresis of the organic matrix in chicken eggshell.