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Two-dimensional electrophoresis1, 2) has been a powerful tool in various fields of life science. The high resolution of two-dimensional electrophoresis provides the capability of detecting minor protein changes which appear with aging, carcinogenesis, disease states and therapies. We developed a new type of two-dimensional electrophoresis3), which was performed on the layers of cellulose acetate membrane. This method had many remarkable properties: (1) it required only a little time to perform; (2) membrane was an easy supporting medium to handle; (3) enzyme activities were detectable after electrophoresis; (4) several replicas of protein map were available for various detection methods, and so forth. However, there was difficulty in using the two-dimensional method to determine quantitative differences in protein spots. Although several groups had reported their methods of twodimensional densitometry4-6) using a minicomputer system, the minicomputer was less accessible than a microcomputer for us. Thus, we devised a microcomputer-aided system for two-dimensional densitometry. In this paper, the method of quantitative analysis of two-dimensional electrophoretogram is discribed and discussed. Materials and Methods

An approach to quantitative analysis of two-dimensional electrophoretogram.