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In this study, the use of Near Infrared Reflectance Spectroscopy (NIRS) as a method to estimate the nutritional quality of Guinea grass (Panicum Maximum) and corn (Zea Mays), was evaluated. These forage species were collected in the Cesar and Sinu valleys of the Colombian Caribbean. Activities included chemical analysis (content of dry matter, ash, ether extract, crude protein, neutral detergent fiber, acid detergent fiber, lignin, non structural carbohydrate and non protein nitrogen as well as the in situ digestibility) on 70 Guinea grass and 193 corn samples; generation of the samples absorption spectra; correlation of the chemical and absorption spectra data for each component (calibration equations) and validation of the calibration equations with 22 Guinea grass and 55 corn samples. Predictions obtained with the NIRS method were similar to the chemical measurements, except for non-structural carbohydrates, which did not relate properly due to overlapping of their spectra with those from cellulose  and from non protein nitrogen because this component involves many compounds (amines, amides, amino acids, peptides that no precipitate with TCA, nucleic acids, nitrites and nitrates), that apparently do not absorb at the near  infrared region tested.

ciencia y tecnología agropecuaria

Aplicación de la Espectroscopía de Reflectancia en el Infrarrojo Cercano (NIRS) para la caracterización nutricional del pasto Guinea y del grano de maíz