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Objective. The effect of cryopreservation on the proximate composition of microalgae Chaetoceros calcitrans was evaluated. Materials and methods. C. calcitrans was cultured and cryopreserved using 5% (v/v) dimethylsulfoxide as cryoprotectant. The freezing was controlled at a rate of 3°C/min, up to -60°C and then the microalgae were immersed in liquid nitrogen (-196°C). After storage in liquid nitrogen, microalgae were rapidly thawed out and subcultured. The percentage of proteins, lipids and carbohydrates was analyzed using absorption spectrophotometry and the organic matter was studied by gravimetric analysis. Results. There was no significant variation between the proximate composition of C. calcitrans cryopreserved and the controls (p>0.05). Conclusion. Our results show that, despite low cell recovery after the preservation of this organism at low temperatures, there is no apparent loss of nutritional characteristics caused by the storing process at low temperatures. RESUMEN Objetivo. En este estudio se evaluo el efecto de la tecnica de criopreservacion sobre la composicion proximal de la microalga Chaetoceros calcitrans. Materiales y metodos. C. calcitrans fue cultivada y criopreservada empleando dimetilsulfoxido al 5% (v/v) como crioprotector. La congelacion fue controlada a una tasa de 3°C/min, hasta llegar a -60°C y luego las microalgas fueron sumergidas en nitrogeno liquido (-196°C). Posteriormente, las microalgas fueron descongeladas de manera rapida y nuevamente cultivadas. Seguidamente se analizo la cantidad porcentual de proteinas, lipidos y carbohidratos, empleando espectrofotometria de absorcion y la materia organica por gravimetria. Resultados. Los resultados indicaron que la composicion proximal posterior a la criopreservacion no evidencia diferencias significativas respecto a los controles no criopreservados (p>0.05). Conclusiones. Los resultados indican que pese a la baja viabilidad post-criopreservacion de C. calcitrans, no hay perdida aparente de la composicion nutricional debida al almacenamiento a bajas temperaturas.

Proximate composition analysis posterior to the cryopreservation of Chaetoceros calcitrans