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We have been studied the characteristics of protein components in gingival crevicular fluid (GCF) with isoelectric focusing technique. The purpose of this study was to investigate the relationship between those proteins and clinical findings such as Gingival Index (Loe &amp; Sillness), Pocket depth, Plaque Index (Quigley &amp; Hein), Gingival Bleeding Index and Bone Index. GCF, serum and mixed saliva collected from the same subject were analyzed, and following results were obtained. The patterns of protein bands in GCF were similar to those in serum but different from mixed saliva, and the pI of main protein band of GCF and serum were 4.9. No apparent difference was observed on the patterns between clinically healthy gingiva and those received periodontal disease. Protein patterns were compared with the before mentioned clinical findings and GCF volume. Main protein bands were observed on cathode side (pI 8.0-9.0) at the patterns against Gingival Index: 0-1 or greater than 2, Gingival Bleeding Index: + or -, Pocket Depth: 0-1 or greater than 2 and Bone Index: 1 or greater than 3. It was suggested that the study of the proteins on cathode side is important.

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Analysis of protein band pattern in gingival crevicular fluid. Character and clinical comparison between healthy subjects and periodontal disease patients.