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A novel efficient Sec-dependent type gene expression vector pAU5 was constructed in the . The vector pAU5 employs the strong promoter  for controlling a constitutive transcription of the target gene, the consensus ribosome binding site (RBS) sequence of  to ensure protein translation, and the efficient Sec-type cgR_2070 signal sequence to mediate protein secretion in the  The pAU5 system is an efficient expression system for the secretory production of the heterologous proteins.

iranian journal of biotechnology

Development of A Novel Gene Expression System for Secretory Production of Heterologous Proteins via the General Secretory (Sec) Pathway in Corynebacterium glutamicum