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Recent studies of Kunkel and his associatesl)-5) have clarified the cloned complementary DNA sequences corresponding to the Duchenne muscular dystrophy (DMD) gene. In the end of 1987, Hoffman et al.~>>7) obtained specific antibodies directed to the proteins encoded by fragments of the mouse DMD gene which is very similar to the human DMD gene. These antibodies recognized a specific protein, named "dystrophin" in normal skeletal and cardiac muscle of both humans and mice, which is absent in two DMD patients and mdx mice. By using these antibodies, Hoffman et al.°) have shown that dystrophin is associated with intracellular membrane fraction, especially the triads, on the basis of Western blot analysis using the pellet obtained by the subcellular fractionation of the mouse and rabbit skeletal muscle. Here we report that the antiserum raised against peptide fragment prepared as predicted from the distinctive region of DMD cDNA stained clearly the surface membranes of normal skeletal muscles of both humans and mice, but did not those of both DMD and mdx skeletal muscles, immunohistochemically. Materials and methods. The peptide fragment was predicted from position 1526 to 1675 on the human cDNA map7} and an extra Tyr was added to the N-terminus. The protected peptide was synthesized by the solid phase method on Applied Biosystems model 430A and deprotected with HF.9) The peptide was purified by preparative reversed phase HPLC. It consists of 51 amino acids, having a molecular weight of 6,267. New Zealand white female rabbit was immunized with intradermal injection of 2 mg of the peptide with Freund's complete adjuvant 3 times every 2 weeks to obtain the antibody. The 4 im frozen sections of the biopsied muscles of 5 patients with DMD and 5 other related neuromuscular diseases and 3 normal controls and 3 mdx (C57BL/lOScSn-mdx) and normal control mice (C57BL/1OScSn) hind limb muscles were incubated with antiserum and stained with fluorescence labeled antibody against rabbit IgG and were observed under fluorescence microscope (Zeiss, Axiophot). Results. As shown in Fig. 1B, the antiserum stained the whole surface

proceedings of the japan academy. series b, physical and biological sciences

Negative immunostaining of Duchenne muscular dystrophy(DMD) and mdx muscle surface membrane with antibody against synthetic peptide fragment predicted from DMD cDNA.