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Twohundredandseventeenticksrandomlycollected fromseventytwodomesticdogs,inMatamorosmunicipality,Coahui- la, theseweretaxonomicallyidentifiedasRhipicephalus sanguineus Latreille. InordertodetermineRickettsiasp. presenceinticksthese weregroupedin 100 poolsformoleculartesting. Engorgedfemale ticksweredissectedtoobtainpelletscontaininginternalorgansand haemolymph.Nymphsandmalesweretrituratedforexposinginternal contentsfromwhichDNAwasextractedbytheCTABtechniqueand subjectedtoPCRtoamplifythegltA genefordetectionofRickettsia.  ThefrequencyforRickettsiasp. was4%(4).Thepresentworkconsti- tutesthefirstrecordofthecausativeagentofthespottedfeverRick- ettsiasp. ininternalorgansofbrowndogtickR. sanguineusthrough  moleculartechniquesinMatamorosmunicipality,Coahuila,endemic regionofthisdisease.

Detección de Rickettsia sp. en la garrapata café del perro Rhipicephalus sanguineus (Acari: Ixodidae) en Matamoros, Coahuila, México