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This study describes sex-steroid cycles of free-living male and female Bolson tortoises at the Mapimi Biosphere Reserve in northeastern Durango, Mexico. Blood samples were taken at biweekly intervals during a 14 month period by jugular venipuncture, and plasma levels of steroids were determined using radioimmunoassays. Hormone levels showed seasonal trends. In females testosterone peaked following emergence, associated with increased sexual receptivity, and estradiol increased after emergence in association with follicular maturation. These two steroids increased with seasonal rains, when follicular enlargement and vitellogenesis occurred. Females emerged from brumation with low levels of progesterone,

Sex-steroids associated with the reproductive cycle in male and female Bolson tortoise, Gopherus flavomarginatus