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Thymusdaenensis is an Iranian medicinal plant with high antibacterial and antioxidant effects. The present study was done to investigate the antibacterial and antioxidant effects of T. daenensis essential oil collected from the Dena mountain, Iran. T. daenensis was collected and immediately transferred to the laboratory. After its approval by an expert person, its essential oil was extracted using the Clevenger apparatus. DPPH free radical scavenging activity and antibacterial effects of various concentration of T. daenensis essential oil were studied. Higher concentrations of the T. daenensis essential oil had higher antibacterial and antioxidant effects. T. daenensis essential oil in a concentration of 1 mg/ml had the highest inhibitory effects on the DPPH radicals (96.27±8.60%). The mean dimeter of the zone of inhibition for tested bacteria had a range of 6.03±0.28 to 19.29±1.57 millimeter. The highest antibacterial effects were seen for the 1mg/ml concentration of T. daenensis essential oil on all tested bacteria and especially on E. coli (19.29±1.57 mm) and K. pneumonia (18.0±1.69 mm). Bacteria were resistant against some of tested antibiotic agents and especially tetracycline, gentamicin and penicillin G. Essential oil of T. daenensis had a higher antibacterial effects on its high concentrations than several types of antibiotic agents. We found that T. daenensis essential oil is a good source for production of natural antioxidant and antibacterial agents.

Antibacterial and antioxidant effects of Thymus daenensis essential oil