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A new UPLC method has been described to determine ketorolac tromethamine in pharmaceutical samples and biofluids. Drug was eluted using a reversed-phase Gemini 3U, C18, 110R (150 × 4.6 mm, 3 μm) column when a mobile phase composition 90:05:05 ( v/v ) consisting CH 3 OH, CH 3 CN and NaH 2 PO 4 under isocratic program.Ketorolac tromethamine was eluted at a short of time 2.02 min. Ketorolac tromethamine isolated from biofluids after liquid-phase extraction. The results were linear up to 10 µg mL -1 with the correlation coefficients greater than 0.999. The sensitivity limits, LOD and LOQ were 0.016 and 0.051 µg mL -1 , respectively. The method is simple, fast and reliable with good specificity and sensitivity and will be suitable for routine analysis of ketorolac tromethamine.

Estimation of Ketorolactromithamine in Phamaceutical Samples and Biofluids by The New Validated UPLC Method