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Hyperleptin affecting oxidation process of N-acetyldiphosphat (NADPH) enzyme which functioned in the metabolic process and redox reaction, resulting oxidative stress. ROS increasing trigger the activation of nuclear factor kappa beta (NF-κβ) which alters redox reaction in the cell through translocation of p50 and p65 dimer to the cell nuclear affecting promoter gen to express chemo taxis factor, monocyte chemoattractant protein-1 (MCP-1). The research aims to observe the quercetin effectiveness on pro-inflammation protein expression decreasing caused atherosclerosis. This is an experimental laboratory experiment using human endothelia cell sample (HUVECs) exposed by Leptin 500ug/mL and incubated for 6 hours then treated with quercetin with dose variation of 0 µM, 50 µM, 125 µM and 625 µM. The NF-κβ activation observed with immune-fluorescence method and MCP-1 level measurement used ELISA technique. Data analysis using Tukey showed the significant decreasing of NF-κβ activation after incubated with quercetin 125 µM (p<0,001) and decreasing level of MCP-1 as 503,40±23,93324 (p<0,001) compared to positive control. The optimal dose of quercetin that significantly decreases MCP-1 level and NF-κβ activation inhibition is 125 µM. Meanwhile, quercetin dose of 625 µM only inhibiting NF-κβ activation but not significantly decreasing the MCP-1 level (p=0,916). The increasing of Quercetin dose showed a significant correlation with the MCP-1 level decreasing (r=-0,498, p<0,001) and NF-κβ activation inhibition (r=0,803, p<0,001).

Quercetin sebagai Penghambat Aktivasi NF-κβ dan Penurunan Kadar MCP-1 pada Kultur HUVECs yang Dipapar dengan Leptin