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[Abstract] Transient expression in tobacco plant (Nicotiana benthamiana) is used to determine the subcellular location of a protein of interest when tagged with a reporter such as green fluorescent protein (GFP), or to mass produce proteins without making transgenic plants. The root tumor bacteria, Agrobacteria, are used to introduce the target gene expression cassette into benthamiana mesophyll cells.

Infiltration of Nicotiana benthamiana Protocol for Transient Expression via Agrobacterium