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1VIB-KU Leuven Center for Brain & Disease Research, Leuven, Belgium; 2Department of Neurosciences and Leuven Brain Institute, KU Leuven, Leuven, Belgium; 3Baylor College of Medicine, Jan and Dan Duncan Neurological Research Institute at Texas Children’s Hospital, Houston, TX, USA; $Present address: Neuroscience Department, Janssen Research and Development, A Division of Janssen Pharmaceutica NV, Beerse, Belgium *For correspondence: joseph.mcinnes@bcm.edu; patrik.verstreken@kuleuven.vib.be

Purification of Soluble Recombinant Human Tau Protein from Bacteria Using Double-tag Affinity Purification