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1. Target cells: Acute Myeloid Leukemia (AML) cell line (U937 or HL60 from ATCC) or AML cells isolated from patients with AML 2. Effector cells: γδ T cells isolated from Healthy volunteer provided by Etablissement 3. Francais du Sang (EFS) or patients 4. Medium : RPMI 1640 (Life Technologies, Gibco®) 5. Fetal calf serum (FCS) heat-inactivated 1 h at 56 °C 6. Chromium-51 (51Cr, 5 mCi/ml) (PerkinElmer) 7. Complete medium = RPMI 1640 medium supplemented with 10% heat-inactivated FCS

Standard 4-hours Chromium-51 (51Cr) Release Assay