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1. Plant growth Surface-sterilized Arabidopsis seeds are planted on ? x modified Hoagland medium (1% agar, 5 mM MES-K, pH 6.0) solidified on 1 ml pipette tip box insert (the part with holes). After germination for 2-3 days under light, the boxes with young seedlings are floated in ? modified Hoagland liquid medium with 5 mM MES-K at pH 6.0, aerated by fish tank water pumps. 2. Extraction of apoplastic wall fluid (AWF)

Extraction of Root Apoplastic Wall Fluid for Apoplastic Peroxidase Activity Assay