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In 1960, Hoseinl' found carnosine and anserine in the rat brain and Pisano et al.," homocarnosine in the rat and beef brain. Tallan et al." reported a chromatographic method for separating these peptides, and a method of using Amberlite IR-120 was published by Moore, Spackman and Stein." A 10 to 20 gm of the animal and the human brains was taken and dropped into an ice cold l %/ picric acid solution of 10 times volume, and after homogenation in a Waring blendor centrifuged for 20 minutes at 4,000 r.p.m. at 0°C. The supernatant was filtered and the filtrate was passed through Dowex 2-X8, 6 cm high resin bed, in order to remove picric acid. The effluent was evaporated in a rotary evaporator to 5 ml at 40'C and the concentrate was applied to a 50 cm high column of Amberlite IR-120. In the chromatogram by this resin homocarnosine and carnosine have the same travel rate, therefore the effluent corresponding to the peak of these peptides was hydrolyzed and rechromatographed and hydrolysis products, which appeared in the chromatogram, were investigated. Table 1 is the results of our experiments on the brain of the various animal species. Anserine has not yet been hydrolyzed, then it might be homoanserine (a dipeptide of r-aminobutyric acid and 1-methyl histidine) as Pisano et al." suggested. In the table 1, the term anserine like substance was used and the value of the substance was calculated as anserine. Table 2 shows the results on the various parts of the human brains which were taken 6 to 12 hours after death. Next, we studied on the metabolism of homocarnosine, but the brain homogenate could not decompose this peptide, it was suggested, therefore,

139. Presence of Homocarnosine and Anserine in the Brain of Various Animal Species