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physical condition was investigated for establishing the optimal regime of brain tumor synchronization as pretreat ment for radiotherapy. Cultured Ce strain was treated for 72 hours with Vincristine, Vinblastine, ACNU, BCNU, 5-FU, Methotraxate, Bleomycin, hyperthemia or microwave irradiation. The cell cycle distribution was analysed by the histograms of pulse-cytophotometry with DNA specific fluorescent stain of ethidium bromide in cell suspen sion. The quantitative evaluation of cell cycle phase was obtained using a computerized mathematical model. Potent synchronization in the G2-M phase was observed in the group treated with Vincristine, Vinblastine, ACNU and BCNU. In the Vincristine and ACNU-treated group, G2-M phase accumulation increased gradually during 72 hrs. Maximum level of G2-M phase was 47.6% of diploid cell population by 72 hrs-contact with Vincristine, and 47.2% by ACNU (control 10%). In the Vinblastine and BCNU-treated group, G2-M phase-accumulation in creased rapidly during 24 hrs. and then the accumulated cells in G2-M phase became dead within 72 hrs. Hy

Pulse-cytophotometric Studies on Experimental Brain Tumor under the Effect of Chemotherapeutic Agents, Microwave Irradiation and Hyperthermia