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An important property of starch is retrogradation which affects the hydrolytic behavior towards the enzyme action. Enhancing the starch with borate increases the viscosity by reacting with the hydroxyl group of the starch which in turn changes the hydrophilicity of the starch. In the present work, starch film is prepared by using borate as gelling agent and subsequently retrograded with temperature treatment of -10C and 10C and 25C over a period of 15 days. Starch film treated with amylolytic enzyme extract and hydrolysis change is studied. Results of hydrolysis are supported with FTIR and TGA techniques. Rate of hydrolysis for starch film at -10C showed significant difference towards enzyme action at 24 hours compared to 10C and 25C treated film. Starch with borate complex improves the retrogradation of the starch film at low temperature and makes starch more susceptible to enzyme hydrolysis. TGA showed that percent weight loss were the lowest for -10C temperature treatment. The difference in decomposition temperature of retrograded starch at 10 and -10C varied from 25C treatment by temperature difference of 5C over the duration of study. It is concluded that starch borate complex improved retrogradation properties at low temperature which makes the film more suitable for amylolytic hydrolysis.

Enzyme Hydrolytic Behavior of Tapioca Starch Borate Complex Under Different Storage Temperatures