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Caffeine is naturally found in plant products such as coffee beans, cocoa beans and tea leaves. In this study, caffeine molecular imprinted polymer (MIP) was synthesized by dissolving 1 mmol caffeine and 4 mmol monomer, methacrylic acid (MAA) and 1mmol ethylene glycol dimethacrylate (EDMA) as the crosslinker and benzoyl peroxide (BOP) as the initiator. MIP exhibits significantly high affinity and selectivity for the target analytes used as the template than for similar molecules, including closely related isomers. The efficiency of extraction using caffeine MIP as sorbents in solid phase extraction (SPE) was evaluated. Good recovery (91.6 %) and low percent RSD (0.9 %) was obtained using the spiked samples (10 µg/mL). The molecular imprinted polymer solid phase extraction (MISPE) technique was then applied in the extraction of caffeine from various coffee samples. Based on the excellent selectivity, MIP is a promising approach for the extraction of compounds from a complex matrix such as herbal extract.

Application of Molecular Imprinted Polymer Solid Phase Extraction (MISPE) in the Extraction of Caffeine From Coffee