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The studies on the alcoholic fermentation with immobilized S. cerevisiae cells on alginate using a bioreactor with stirred beds of biocatalysts indicated that the diffusion inside the biocatalyst particles attenuates or avoids the inhibitory effects, preserving the microbial activity. Using a mathematical model adequate for ethanol formation in heterogeneous systems the kinetic parameters V, KI and K'M have been estimated. Therefore, the obtained values of V and K'M were found to be higher than those corresponding to the homogeneous media under ethanol inhibition, but lower than those for homogeneous media without inhibitory fenomena.

Kinetic Studies on Alcoholic Fermentation Under Substrate Inhibition Conditions Using a Bioreactor with Stirred Bed of Immobilized Yeast Cells