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A two-dimensional gel electrophoresis (2D-GE) and capillary isoelectric focusing (cIEF) were performed sub- sequently to analyze protein mixtures from laboratory animal tissues (liver, muscle, and mammary gland). Both tech- niques were compared and evaluated. It was found that cIEF can be a valuable alternative in comparison to 2D-GE. Espe- cially considering the goals associated with the determination of far the separated proteins, cIEF possesses perspective analytical advantages.

PI-Based Separation of Tissue Proteins Realized with the Use of Two-Dimensional Gel Electrophoresis or Alternatively Capillary Isoelectric Focusing