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Small interfering RNAs can induce RNA silencing throughout plants owing to their ability to move through the phloem. Although microRNA (miRNA), another noncoding small RNA that regulates protein-coding RNAs through transcript cleavage or translational inhibition, has also been detected in phloem, there are few reports of its long-distance movement via phloem. We investigated whether miR172 molecules can move systemically from source tissues to sink tissues in Nicotiana benthamiana. Results of agro-infiltration and micro-grafting experiments indicate that miR172 can move long distances.

miR172 Can Move Long Distances in Nicotiana benthamiana