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Specimens containing Rheumatoid factor (RF) were examined for possible interference in Prolactin (PRL) measurement. PRL levels following polyethylene glycol (PEG) treatment were significantly lower than in untreated RF- positive samples (p<0.001; n=43) and spurious hyperprolactinemia was indicated in 7 percent. Gel filtration chromatogra- phy suggested that the PRL-like immunoreactivity was from assay interference rather than a macroPRL complex and that PEG treatment successfully removed the interference. It is important that laboratories consider explanations other than macroPRL when interpreting PRL results following PEG treatment.

Prolactin Immunoreactivity in Rheumatoid Factor-Containing Specimens: Is it Prolactin, Macroprolactin or Assay Interference?