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Phytase and phytase gene from Aspergillus ficuum (A. ficuum) were used in this study. The results showed that phytase activity reached the peak of 0.17 U/g after 4 d incubation in solid medium for A. ficuum; the optimum pH and temperature of phytase were 2.5 and 50 o C, respectively. A 1.4-kb DNA containing the coding region of phytase gene was isolated and inserted into the expression vectors of pIA 8 and pGAPZA, which were transformed into E. coli (Top 10). The maximal phytase activities in the supernatant and cells were 2.31 and 9.04 U/ml for the E. coli with pIA 8, 8.04 and 2.93 U/ml for the E. coli with pGAPZA, respectively. It was concluded that the recombinant of pIA 8-phytase could ex- press intracellular phytase, while the recombinant of pGAPZA-phytase could express extracellular phytase. The molecu- lar weight of phytase protein was 54.61 kDa.

Phytase Expressed by pIAβ8 and pGAPZαA Vectors and Analysis of its Biochemical Characters