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This paper shows how fish samples could be directly injected into a chromatograph, without any previous extraction, using micellar liquid chromatography, taking as an example the determination of two biogenic amines: putrescine and tyramine, derivatized with 3,5-dinitrobenzoyl chloride. First, the method has been optimized, using an interpretive model, and the best conditions for analysis were: mobile phase containing 0.15 M SDS-6% (v/v) butanol-pH 7, C18 column (125 x 0.46 mm, 5 μm particle size), UV detection of derivatives set at 260 nm. Second, the method has been validated using spiked samples following the guidelines proposed by the US Food and Drug Administration: linear in the range from 0.5 to 1000 ppm (r2>0.999), detection limits of 30 and 40 ppb for putrescine and tyramine, respectively, a 500 ppb limit of quantification in both cases, intra- and inter-day precision (RSD) below 2%, and accuracy between 99.5% and 102.7%. The method is adequate for routine analysis in the determination of these compounds in fish sauces

Determination of Putrescine and Tyramine in Fish by Micellar Liquid Chromatography with UV Detection Using Direct Injection