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The traditional approach to assessing eicosanoids in cell cultures by liquid or gas chromatography coupled to mass spectrometry involves time-consuming procedures such as derivatisation, degradation, solid-phase extraction, liquid- liquid extraction, thin layer chromatography and sometimes, combination of all these procedures prior to the injection of the sample in the chromatography system. The performance of a method that allows the direct injection of redissolved cell culture media into a single-column liquid chromatography instrument coupled to mass spectrometry is evaluated. The ex- ternal standard calibration curves were linear between 1-50 ng/ml for Williams' medium E and L-15 medium. The limit of detection and quantification were 0.5 and 1 ng/ml in both media respectively. The recovery values were 98.6% for Wil- liams' medium E and 100.3% for L-15 medium. The internal standard method was not used for quantitative purposes due to the variability of the response factor. The proposed method has potential for broad implementation in monitoring bio- markers in cell cultures.

Direct Injection of Redissolved Cell Culture Media into a Single-Column Liquid Chromatography Coupled to Mass Spectrometry for the Measurement of PGE2