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LBL-7487 LOW TEMPERATURE ELECTRON MICROSCOPY ANo ELECTRON DIFFRACTION OF THE PURPLE MEMBRANE OF HALOBACTERIUM BALOBIUM University of California Berkeley, California 94720 ABSTRACT The structure of the purple membrane of Halobacterium halobium has been studied by high resolution electron microscopy and electron diffraction, primarily at low temperature. The handedness of the purple membrane diffraction pattern with respect to the cell membrane was deter mined by electron diffraction of purple memb-,nes adsorbed to polylysine. A new method of preparing frozen specimens was : i d to preserve the high . e resolution order of the membranes in the electron iiicroscope. High resolution imaging of glucose-embedded purple me. .>rnnes at room temperature was used to relate the orientation of the diffraction pattern to the absolute orientation of the structure of the bacterior!:odopsin molecule. The purple membrane's critical dose for electron beam-induced damage has been measured at room temperature and at -120°C, and has been

Low temperature electron microscopy and electron diffraction of the purple membrane of Halobacterium halobium