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During the first year of this contract great efforts were made to develop methods for (1) characterizing bacteria from soil and sediment, (2) evaluating the ability of single and mixed soil bacterial isolates to, (a) bioconcentrate, (b) biodegrade and/or (c) precipitate inorganic and organic pollutants and (3) expanding current concepts for treating waste in aqueous (i.e. biological waste treatment system) and solid media (i.e. in situ soil (soil) treatment system). The development of the above methods are in the final stages of completion and we have as a result of these efforts isolated from soil (1) a mixed culture which precipitate toxic metals (i.e. mercury cadmium, lead etc.) and (2) single isolates which bioconcentrate a variety of toxic metals. Methods for screening soil bacterial isolates for their ability to concentrate, degrade and/or precipitate environmental pollutants have been developed. The development of those methods will allow the staff at ORRI to quickly screen hundreds of samples in our attempt to isolate bacteria capable of degrading, concentrating and/or precipitating inorganics and organics in aqueous and solid waste. The results of these studies are summarized below

osti oai (u.s. department of energy office of scientific and technical information)

Developing biological and chemical methods for environmental monitoring of DOE waste disposal and storage facilities. Progress report, April 1, 1985--October 30, 1985